Standardization, Antidaibetics And Antioxidant Assessment Of Hydroalcoholic Extract Of Abies Webbiana Fruit With Isolation Of Ferulic Acid
DOI:
https://doi.org/10.64252/8phaq332Keywords:
Abies webbiana, hydroalcoholic extract, ferulic acid, antioxidant activity, phytochemical screening, HPTLC, DPPH, pharmacognostical evaluation.Abstract
The present study aimed to evaluate the pharmacognostical, phytochemical, and antioxidant properties of Abies webbiana fruit, with an emphasis on its hydroalcoholic extract. Pharmacognostical analysis confirmed the quality of the crude drug through parameters such as ash values and loss on drying. Extraction yield and solubility studies indicated that the hydroalcoholic extract was richer in polar phytoconstituents compared to the petroleum ether extract. Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, glycosides, tannins, phenolics, and terpenoids in the hydroalcoholic extract. Quantitative assays showed a high content of total phenolics (154.44 mg GAE/g) and flavonoids (98 mg RE/g), which correlated with strong antioxidant activity in DPPH, reducing power, and superoxide scavenging assays. TLC and HPTLC confirmed the presence of ferulic acid,. The study concludes that the hydroalcoholic extract of Abies webbiana fruit, rich in phenolic compounds like ferulic acid, exhibits significant antidiabetic and antioxidant potential, supporting its traditional use and potential pharmaceutical applications.
