Simultaneous Equation Technique For The Estimation Of Quercetin And Curcumin By Uv- Visible Spectrophotometry

Abstract

A simple and repeatable U.V. spectrophotometric approach was created and validated for the quantitative measurement of Curcumin (Cr) and Quercetin (Qr). According to the ICH criteria, by utilizing the parameters, namely accuracy, Limit Of Quantification (LOQ), linearity, Limit Of Detection (LOD), and recovery, the technique was validated. Now, by utilizing a quick, sensitive, exact, and affordable spectrophotometric approach in the UV area, Quercetin and curcumin concentrations in formulations might be determined. By utilizing methanol and techniques that adhered to Beers-law Lambert's at the concentration levels used for analysis, the samples were made. Therefore, for accurately measuring the amount of an active marker component in raw drugs and formulations, the suggested approach may be wielded. At 379 and 416.5 nm, the most absorbance was shown by the Quercetin and Curcumin, correspondingly. Bear Lambert’s law is obeyed by each ingredient within the concentration of 2-10 µg mL-1 for Quercetin and Curcumin. For Quercetin, the LOD and LOQ values attained are.38 µg mL^{- 1} and 4.20 µg mL^-1. Also, the LOD and LOQ values attained for Curcumin are 0.300 µg mL^-1and 0.9102 µg mL^-1, correspondingly.