Susceptibility for antibiotics and molecular studies to detect the Lipase gene in Propionibacterium acnes, isolated from a patient with acne.

Abstract

Propionibacterium acnes( P. acnes), a usual inhabitant of the human skin, is anaerobic, Gram-positive, and rod-shaped, andit plays an important role in acne. Isolate bacteria is cultured on selective media (3-7)days anaerobically 5( 9.1 %) isolates from  were positive as P.acnes; since it can form a biofilm, which makes it more resistant to the antibiotic, therefor; biofilm assay by microtiter plate method, and all isolated were positive and robust; antibiotic susceptibility has been proceeded by the procedure  Kirby-Bauer on the Mueller Hinton agar and chose 15 types of antibiotics the result shows that all (100%) P. acnes bacteria were resistant to ceftazidime (30µg),amoxicillin(25µg), ampicillin (10µg),ciprofloxacin(5µg), clindamycin (10µg), and pencillin (10 µg ); on other hand, all 100% sensitive to doxycyclin(30 µg), azithromycin (10µg), rifampin (5µg), amikasin(30µg), azitreonam(30µg), impenine(10µg)  moreover, confirm identification by polymerase chain reaction (PCR) technique by the sequence of 16 S rRNA and one of important virulence factor (lipase ) play important factor in pathogensisand the first target of treatment coded by gehA. The PCR amplification products were disclosed by electrophoresis. The sizes of the amplicons 16S rRNA and Geh A size product gene were determined by comparison to the 1000 bp allelic ladder.The results showed that all isolated samples were 5(100%) positive for 16S rRNA, and the lipase gene was positive in 3 out of 5 cases (2 of them were males) and negative in the remaining 2 cases, who were from both sexes with a non-significant difference P<0.005 (P=1.000).Considering the P-value less than 0.005, a significant