Effect of Adding Extracted Conjugated Linoleic Acid (CLA) on Hypercholesterolemic Female White Rats and Its Comparison with Commercial Conjugated Linoleic Acid

Abstract

The results of dosing and the effect of different concentrations of conjugated linoleic acid (CLA) on hypercholesterolemic female white rats fed a high-fat diet indicated a significant decrease in cholesterol level to 78.46 mg/dl at 2% CLA concentration, compared to the group fed a high-fat diet without CLA, which showed an elevated cholesterol level of 119.67 mg/dl. Triglyceride (TG) levels decreased across all concentrations compared to the high-fat treatments, reaching 78.88 mg/dl at the 2% concentration, while they were 81.82 mg/dl and 94.80 mg/dl at the 0.5% and 1% concentrations, respectively. In the control group, the level was 32.1 mg/dl. Administration of conjugated linoleic acid (CLA) at varying concentrations resulted in notable effects on HDL and LDL cholesterol levels in laboratory animals. HDL concentrations increased across all treated groups, with the highest level observed at the 2% concentration (45.36 mg/dl), compared to 42.70 mg/dl and 41.40 mg/dl at the 1% and 0.5% concentrations, respectively. In contrast, the control group recorded a lower HDL value of 32.1 mg/dl. LDL levels, on the other hand, decreased significantly at the 2% concentration, reaching 44.10 mg/dl, compared to higher levels at the lower concentrations. Furthermore, the hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) showed reduced activity at the 2% CLA concentration, with values of 40.98 IU/L and 55.18 IU/L, respectively.