Metabolite Extraction And Functional Annotation Of Cyclea Peltata From Western Ghats: Insights Into Ethnopharmacological Potential

Abstract

Cyclea peltata is very important medicinal plant which is traditionally used in Vythiri thaluk, Wayanad district, Kerala, India against different diseases in human and animals. The objective of this study was to evaluate antimicrobial activity and characterize the chemical constituents in the extracts of the leaves of Cyclea peltata by using modern techniques like High Performance Liquid Chromatography (HPLC) and Liquid Chromatography–Mass Spectrometry (LC/MS-MS). The evaluation of antimicrobial activity of Cyclea peltata was determined by disc diffusion method. The extracts showed different degree of inhibitory potential against all the tested bacteria such as Escherichia coli and Pseudomonas aeruginosa and two gram-positive strains of Bacillus subtilis and Staphylococcus aureus. LC/MS-MS analyses showed that majority of identified compounds in Cyclea peltata are alkaloids, and flavonoids.The high percentage of compounds that were identified in the extracts of which are chemically and biologically important. In HPLC fingerprint Cyclea peltata showed total 5 major peaks to attribute as novel metabolite and active ingredients. Therefore, the identified good number of chemical compounds from methanol extract of the leaves of Cyclea peltata have many ecological benefits for different ailments. Result from this study suggested that the extracts of the leaves of Cyclea peltata could be used as a natural antimicrobial agent.