Gold Nanoparticles As Gastrointestinal Prevention For Escherichia Coli-Induced Colitis In Rats

Abstract

Background: Colitis is worldwide distributed disease characterized by abnormal immune responses .Gold nanoparticles (GNPs) are recently used in medical field because their unique properties and have Low toxicity compared to  other metallic .This study aimed  to investigate the gastrointestinal protective ability of orally administrated Gold nanoparticles against E.coli induced ulcerative colitis in rats. Methods: E.coli isolated from cow's milk with mastitis and used to induce ulcer in rats. Waster albino rats was administration orally GNPs at two doses 5µg/Kg & 10µg/Kg as a prevention factor against E.coli  induced ulcer compared with ulcer group and group administration omeprazole and evaluated the immunological effect of GNPs measured the level of SOD, MDA, TNF –alpha, TGF-β and PGE2 by ELISA methods from the blood rats. Results: The result shown that  GNPs have protective effect against induced ulcer as dose dependent manner and have antioxidant properties through elevation of the SOD level  and decrease the level of MDA compared with ulcer and omeprazole groups . Although the GNPs modulating the immune response by lowering the level of TNF –alpha and the level of transforming  growth factor (TGF-β), and PGE2 significant compared with ulcer and omeprazole groups.   Conclusion: Gold nanoparticles has the effective ability of gastroprotective against bacterial induced ulcer through the enhancement antioxidant enzyme and modulation  the proinflammatory as TGFb and PGE lead to decreased tissue injury and inflammatory cytokines TNF-alpha.