Phytochemical Profiling Of Curcumin, Quercetin, And Catechin In Plant Extracts Using A Novel Hplc-Dad Technique

Abstract

Abstract:

Background: Curcumin, a compound of Curcuma longa, quercetin from Allium cepa, and catechin from Acacia catechu were measured simultaneously. Curcumin, quercetin, and catechin were obtained through the development and validation of a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.

Objective: To develop a sensitive, precise, and ICH-compliant HPLC method for simultaneously determining curcumin, quercetin, and catechin in plant extracts.

Methods: HPLC system (Agilent 1100) that consists of a Diode Array Detector (DAD) and the chromatographic separation was carried out using Inertsil C18 column (250 x 4.6 mm, 5 The mobile phase consisted of methanol and phosphate buffer in the ratio of 80:20 v/v and the the flow rate was 1.0 mL/min. The detection wavelength was 280 nm for curcumin and 254 nm for quercetin, catechin, and 274 nm. The method was linearity-validated, precision-validated, accuracy-validated, limit of detection (LOD), and limit of quantification (LOQ) according to ICH Q2(R2) requirements.

Result: Curcumin, quercetin, and catechin showed retention times of 17.1, 3.1, and 6.1 minutes, respectively. The analysis procedure was very precise (%RSD < 2%), excellent linearity (R 2 > 0.980), and recovery rates of 100-102%. LODs of curcumin, quercetin, and catechin were 0.004, 0.020, and 0.003ug/mL, respectively.

Conclusion: The developed RP-HPLC is simple, robust, and reliable for the simultaneous determination of curcumin, quercetin, and catechin in herbal preparations. It can be used in quality control, standardization, and phytopharmaceutical studies.