Isolation And Identification Of Fatty Acids In Serum Of People With Obesity

Abstract

The study focused on obesity as a risk factor associated with all common and prevalent diseases. The isolation and identification of fatty acids in human serum is crucial for diagnosing essential fatty acid deficiencies, fatty acid metabolism disorders, and understanding lipid risk at the population level. In most cases, total fatty acyl analysis is performed using various methods such as gas chromatography (GC). The basic principle of this technique involves the use of acid- or base-catalyzed reactions to convert fatty acyl chains from intact lipids and non-esterified fatty acids into volatile FA methyl ester (FAME) species that can be detected using gas-coupled flame ionization detection (GC-FID) or mass spectrometry (GC-MS). Using various chromatographic parameters (including column materials) and detection methods, fatty acid analyzers can be monitored with multiple resolutions. Consequently, analysis times are increased according to these principles. In our study, five obesity-related fatty acids were isolated: alpha-linolenic acid, linoleic acid, oleic acid, palmitic acid, and stearic acid. Which has proven its direct relationship between obesity