Inhibitory Effect Of Rubus Ulmifolius Leaf Extract On Alpha-Amylase Activity: Phytochemical Profiling And In Vitro Evaluation For Diabetes Management

Abstract

Diabetes mellitus is a chronic metabolic disorder with increasing global prevalence. Controlling postprandial hyperglycemia by inhibiting digestive enzymes such as alpha-amylase is a validated therapeutic approach. Natural inhibitors from plants are valued for their safety and multifunctional bioactivity. This study aimed to evaluate the alpha-amylase inhibitory potential of an aqueous extract from Rubus ulmifolius leaves, known in traditional medicine for antidiabetic properties. Extraction by successive hexane followed by methanol/acetone reflux yielded a rich polyphenolic profile including flavonoids and tannins. The extract inhibited alpha-amylase activity in vitro with an IC50 of 1.80 mg/mL. Comparative analysis with the standard inhibitor acarbose and a detailed phytochemical assay corroborate the potential of R. ulmifolius as a complementary antidiabetic agent. Further in vivo and mechanistic studies are recommended.