Lipid Extraction By Ozonolysis From Scenedesmus Sp. Followed By Purification Using Column Chromatography

Abstract

Microalgae are gaining attention as a promising source of various bioproducts, ranging from biofuels to essential food components, due to their potential in multi-product biorefineries. They offer several advantages over traditional food crops, such as higher biomass productivity, reduced land requirements, and the ability to grow on non-arable land. The extraction of their lipid content can be achieved through more eco-friendly options, such as ozonolysis and methanol. These lipids can then be utilized for biodiesel production through esterification or for the consumption of essential fatty acids. This study focused on extracting lipids from Scenedesmus sp. biomass using methanol with the assistance of ozonolysis, followed by purification with adsorbents in a chromatographic column. Results show that product formation in the ozonation extraction process, microalgae lipid extraction, and yields (Ozonation for 20 minutes at 20˚C, 40 minutes at 40˚C, and 60 minutes at 60˚C). Among the ozonation of the mixture for 20 minutes + 20˚C produced several compounds (2-pentadecanone, 6, 10, 14-trimethyl; n-hexadecanoic acid (also known as palmitic acid); phytol and octadecanoic acid. Ozonation for 40 minutes +40˚C, and 60 minutes +60˚C  clearly produced 3 main compounds (2 pentadecanone, 6, 10, 14-trimethyl; palmitic acid; stearic acid, ).