Assessment the activity of Propolis loaded Nanoparticles against Staphylococcus aureus isolates from tonsillitis Patients

Abstract

Objective: Tonsillitis is inflammation of the pharyngeal tonsils, predominantly affects the pediatric demographic, with a notably elevated incidence rate observed in children. Methods: 154 samples from pharyngeal tonsils  had been collected from patients who had Tonsillitis.  The Samples were obtained from Al Zahraa Teaching Hospital   in Wasit City, Iraq. During the period from October ,2024 to March,2025. then methicillin-resistant Staphylococcus aureus were isolated by traditional methods . High-shear homogenization and ultra-sonication methods were used to prepare LNPs-PL. The manufactured formulation's encapsulation efficiency, loading capacity, and physicochemical characteristics were evaluated. Scanning electron microscopy (SEM) was used to analyze the morphology of LNPs-PL (SEM). Using GC/MS analysis, the chemical composition of the tested normal oil and LNPs-PL was assessed. In vitro studies were conducted to compare the antibacterial activity of LNPs-PL and regular PL.

Results:  the prevalence  of MRSA isolates were 37(58.73%) .the result of The antimicrobial activity of LNPs-PL showed increase the level of inhibition on the MRSA bacteria to (36 mm) comparative to (14 mm) with propolis (PL) Highly significant difference (P<0.01). LNPs-PL showed significantly larger inhibition zones vs. PL.

Conclusion: Based on the results of the study, modifications should be made in the loading capacity, encapsulation efficiency, and physicochemical properties. Our findings confirmed that SLNs are effective PL carriers for controlling bacterial and fungal diseases and further research is needed on them.