Cytotoxic and Apoptotic Effects of Cymodocea Serrulata Extracts on MDA-MB-231 Breast Cancer Cells

Abstract

Cymodocea serrulata is a seagrass that grows in the intertidal region of marine areas. It was analyzed for its efficiency as a source of phytochemical compounds as the potential medicines in the future. The ethanolic extracts of the samples were tested for in vitro cytotoxicity using MDA MB-231 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5- 2,5-diphenyltetrazolium bromide (MTT) assay, Mitochondrial Membrane Potential assay, DNA ladder assay, and Ethidium Bromide/Acridine Orange assay. The absorbance increased with a decrease in the concentration of the selected samples. The DNA ladder assay showed digestion of genomic DNA when cancer cells were treated with plant extracts. Human cancer cell lines incubated with plant extracts exhibited apoptotic DNA fragmentation profiles. This result clearly shows tha it is effective in inducing cytotoxicity by interacting with nuclear DNA