Increased Concentration Of Active Substances That Kill Cancer Cells In Rosemary Plants (Rosmarinus Officinalis L.) By Spraying Growth Regulators

Abstract

The study was carried out in the Department of Horticulture, Faculty of Agriculture, University of Baghdad, Abu Ghraib. Rosemary seedlings, six months old and sourced from a private nursery in Baghdad, were selected based on uniformity in vegetative growth and height. The seedlings were transferred to plastic containers with a diameter of 25 cm and a weight of 10 kilograms, utilizing a soil mixture of river sand and peat moss in a ratio of three to one. Optimal conditions were maintained, including continuous irrigation, hoeing, and weeding. Samples from the agricultural medium were collected to assess the effects of salicylic acid and cytokinin for measurement purposes, with the experiment designed according to a completely randomized block design. RCB as an empirical study comprising 48 treatments 4 × 4 × 3, where the initial factor comprised four concentrations of volatile oil extraction, involved the application of growth regulators (cytokinin and salicylic acid) at cytokinin concentrations of (0, 50, 100, 150) mg per soil. The third factor comprised four amounts of salicylic acid: 0, 100, 200, and 300 mg. The most significant findings indicated that the triple interference resulted in a notable change across all features, with cytokinin at a concentration of 150 mg/l and salicylic acid at 300 mg/l. The maximum output of volatile oil from the plant reached 317 mg per plant in contrast to the control treatment.