Hemolymph Peptide Fraction Of The Algerian Mussel, Mitylus Galloprovincialis (Lamarck), Reduces Carragenan-Induced Inflammation In Rats

Abstract

In addition to their nutritional value and effects on well-being, mollusks in general and mussels in particular are a potential source of several bioactive compounds that have gained much more importance in the last decade due to their protein richness and their various uses as nutraceutical, pharmaceutical, therapeutic and functional food agents. In this study, the in vivo anti-inflammatory activity of Hemolymph Peptide Fraction (HPF) extracted from the hemolymph of the Algerian blue mussel, Mytilus galloprovincialis was evaluated on one hand by measuring the 1% carrageenan-induced edema in plantar fascia of left hind paw of rats, and on the other hand by the dosage of C-reactive protein (CRPr) and fibrinogen, as well as by the histological examination of the inflamed paws. The obtained results evidenced a progressive increase in the volume of plantar edema following the injection of 1% carrageenan, reaching its maximum threshold 4h thereafter in the negative control group (3.21 ± 0.006 mm). The two groups treated with the different concentrations of HPF (30 and 60 mg/kg body weight), showed a highly significant decrease (p < 0.001) in edema, starting 4h after the injection. HPF anti-inflammatory activity registered  at 30 and 60 mg HPF/Kg body weight results in a percentages of inhibition (PI %) of carrageenan-induced edema in the Wistar rat paw of about 28.22 ± 0.31% and 42.74 ± 0.15%, respectively ; whereas CRPr and fibrinogen concentrations, recorded with the same HPF concentrations were 52 and 39 mg/L, and 6.5 and 4.5 g/L, respectively.