Antioxidant Activity Of Isolated Flavonoid Compounds From Leaves Of Euphorbia Neriifolia And Mentha Spicata

Abstract

This study evaluated the antioxidant activity of isolated flavonoid compounds from the leaves of Euphorbia neriifolia and Mentha spicata. Leaf extracts were prepared using successive solvent extraction, and flavonoid fractions were purified through chromatographic techniques and characterized spectroscopically. Antioxidant potential was assessed using in vitro assays, including DPPH. The results showed that both plants exhibited notable free radical scavenging activity in a concentration-dependent manner, with Mentha spicata displaying comparatively stronger antioxidant effects. A positive correlation was observed between total flavonoid content and antioxidant efficacy, confirming the role of these compounds in reducing oxidative stress. The findings suggest that flavonoids isolated from E. neriifolia and M. spicata may serve as promising natural antioxidants for future phytopharmaceutical applications.