Biogenic Silver Nanoparticle from Citrus Bergemia Essential Oil: Synthesis, Characterization and Antibacterial Assessment

Abstract

Objective: The proposed Research work mainly emphasis on Synthesis, characterization of Biogenic Silver Nano-particle from citrus bergemia for various anti-microbial infectious condition. By considering alleged advantages for the environment and human health, natural preservatives as opposed to synthetic ones are becoming more and more popular for preserving the quality and safety of food. Bergamot oil a plant essential oil and extract which contain a high concentration of flavonoids such as neoeriocitrin, neohespiridin, and naringenin are primary preparation employed Realizing the full potential of essential oil derived bioactive compounds in the fight against bacterial infections require an understanding of their chemical variety and mode of action.

Method: Glycyrrhetenic acid, (GA-18), Ursolic acid, Tween 80, PEG-400 and (0.01N) AgNO₃ were selected to prepared antibacterial silver nanoparticle. The silver nanoparticle was prepared by using micro-emulsification technique by using high speed homogenization and then it is transferred for drying purpose to allow free flowing Nano-particle.

Results and Conclusion: Particle size, PDI and zeta potenical measurment, Morphological Evaluation (Field Emission Scanning Electron Microscope (FE-SEM)), FTIR Spectroscopy analysis, Antimicrobial study were used to evaluate prepared silver Nano-particle. Particle size, PDI and zeta potenical measurment gives significant particle size value of 151 nm , 0.300 PDI and 34mV , Significant in-vitro growth suppression of bacillus substillus was demonstrated by the silver nano-particle. Different zone of inhibition(mm) were recored for different sample concentration, as per reported data generated Bergamot oil containing microemulsion based Silver Nano-particle shows considerable antibacteial effect.