Antifertility studies of the flower extract of Tecomella undulata in male albino rats with special reference to testicular cell population dynamics

Abstract

Aim of present research work was to study the antifertility properties of Tecomella undulata ethanolic extracts of flower in male albino rats. Fertile male rats were divided in four treatment groups each having 5 animals. Prepared flower extract was orally administered at dose level of 500 mg/kg body weight for 60 days and TP was administered intramuscularly at dose level of 0.01 mg/day for 30 days. After completion of the dose schedule autopsy was done under mild anesthesia. Different fertility parameters were studies such as reproductive organ weight, sperm motility and density, testicular population count and histology. Statically significant (P≤ 0.001) decrease in weight of reproductive organs in treated groups in comparison to control was observed. Sperm Motility (Cauda) and Sperm density (Cauda and testes) was significantly reduced in all treated groups incomparison to control group. In comparison to control group, degenerative changes and arrest in spermatogenesis was observed in histology of testis of all treated group treated with extract. Reduction in spermatogonia, primary and secondary spermatocytes, spermatids, immature and mature leydig cells was noticed in treated groups (II and III) while regaining in these parameters in group IV was noticed as compared to control. From all research findings we come to conclusion that flower ethanolic extracts of Tecomella undulata with TP has reversible antifertility properties in male albino rats and can serve as oral contraceptive for male without any negative impact.