The Beneficial Impact Of Nano-Piperine On Hormonal Balance And Antioxidant Levels In Female Wistar Rats

Abstract

A research investigation evaluated the beneficial advantages of nano-piperine (administered at 20 mg/kg of body weight) on reducing sodium benzoate's (100 mg/kg of body weight) damaging effects on female hormones and particular antioxidants.  The experiment involved forty Wistar rats of female gender at 120 days old with a weight range of 200±10 g which were organized randomly into four equal treatment groups for four weeks. Distilled water was given to the control group (C) in a one-cc solution.  A sodium benzoate substance served as the treatment agent for group T1.  Nano-piperine constituted the second treatment protocol that the T2 group received.  The experimental group receiving treatment T3 received both sodium benzoate and nano-piperine combined within a single dosage.  The research trial culminated with the execution of female subjects followed by heart blood extraction for tests measuring serum LH, FSH, oestrogen concentration together with MDA, CAT and GSH levels.  Measures of LH, FSH, and Oestradiol showed significant reduction in T1 while T2 and T3 presented higher levels than the control group according to the obtained findings (p<0.05).  The concentrations of MDA increased remarkably in T1 rats and simultaneously there was a significant decrease in CAT and GSH levels yet T2 and T3 rats exhibited opposing results.  The research shows that nano-piperine functions as a protective agent which counteracts sodium benzoate-induced harm to female hormones together with antioxidant balance in adult female Wistar rats.