Recombinant Cyclodextrin Glycosyltransferase: A Bibliometric Review Of Bioprocessing And Downstream Technologies

Abstract

The production of broad-use cyclodextrins in food pharmaceuticals and chemical industries depends on the critical biotechnological enzyme Cyclodextrin glycosyltransferase (CGTase). Scientists have centered their research on recombinant CGTase production because both process optimization and large-scale manufacturing requirements are essential for industrial deployment. This research paper evaluates the progress and difficulties which emerged during the bioprocessing and purification procedures of recombinant CGTase. The investigations into E. coli expression system optimization with fermentation strategies form primary research topics which include protein engineering and efficient purification techniques for heat-stable protein production. After notable achievements have been made the field faces additional obstacles that need resolution like increased large-scale production capacity alongside improved stability under heat conditions and more affordable manufacturing expenses. The research demonstrates how recombinant CGTase finds application in industrial sectors for modifying cyclodextrins used in pharmaceutical drugs as well as food manufacturing processes. Research on recombinant CGTase will concentrate on scale-up difficulties as well as enzyme stability improvements and economic purification techniques to achieve its full commercial potential.