Using Of Phenylalanine To Increase The Anthocyanin Compounds Content Of The Local Variety Of Black Rice And It's Oxidative Activity

Abstract

A field experiment was conducted during the summer season of 2021 at the rice research station in Al-Mishkhab, with the aim of improving the proportions of active compounds with a medical effect among the genotypes used for black rice.The experiment was conducted according to a completely randomized block design (RCBD), in a split-plot arrangement with three replicates. The amino acid Phenylalanine treatments were randomly distributed in the main plots, 50, 100, and 150 mg.L^-1, in addition to the comparison treatment (spraying distilled water only(In the sub plot, the genotypes of Goura, Local and Chakhao black rice were distributed.The results obtained showed that the Goura genotype was significantly excelled, giving the highest averages in the number of branches per plant, the number of total panicle, and the weight of 1000 grain. The total grain yield and the percentage of free radical suppression amounted to 114.77 cm², 32.34 cm², 16.96 tillers .plant^-1, 15.12 panicle.plant^-1, 22.83 g, 5.86 tons.ha^-1, and 65.59%, respectively. While the Local genotype gave the highest averages for total anthocyanins, Cyanidin 3-O-glucoside, Peonidin 3-O-glucoside, Cyanidin, Delphinidin, Peonidin, and Malvidin. The total antioxidant and carbohydrate capacity amounted to 53.32 mg.kg-1 dry weight, 27.88 mg.kg^-1 dry weight, 7.11 mg.kg^-1 dry weight, 5.34 mg.kg^-1 dry weight, and 3.84 mg. kg^-1 dry weight, 1.28 mg.kg^-1 dry weight, 1.24 mg.kg^-1 dry weight, 60.06 mg.kg^-1 dry weight, and 54.68%, respectively.The results also showed the significantly excelled of spraying the amino acid Phenylalanine at a concentration of 50 mg.L^-1 by giving the highest averages for the yield traits, its components, and all the active compounds.