Metagenomics of Gut Microbiota in Diabetic Animal Model Treated with Neeradimuthuvallathy Mezhugu - A Herbomineral Siddha Formulation

Abstract

Diabetes, a chronic metabolic disease marked by high blood glucose levels, has become an epidemic globally. Despite their effectiveness in many situations, current treatment methods have drawbacks, such as the establishment of medication-resistant diabetes, which is commonly accompanied by insulin resistance, negative side effects, and the development of drug resistance. ‘Siddha’, one of the ancient Indian Systems of Medicine, has many drugs in use that treat diabetes effectively. This study examined the effects of Neeradimuthuvallathy Mezhugu (NM), a Herbomineral Siddha formulation, on the gut flora of diabetic rats. NM had a significant antidiabetic activity in the animals and also a more notable beneficial effect on the gut microbiota than the common anti-diabetic medication glibenclamide. Rats with diabetes showed decreased microbial diversity and an altered bacterial composition, with more potentially hazardous bacteria and fewer helpful bacteria.  Beneficial bacteria including Lactobacillus and Bacillus were enriched; microbial diversity was greatly increased, while the number of harmful bacteria was decreased after NM therapy. With the high NM dosage, Group 6 (HD) animals showed the most noticeable results, with the most diversity and enrichment of good bacteria such as Bacillus celluloses and Lactobacillus acidophilus. These results imply that NM could be beneficial for the treatment of Diabetes by altering the gut flora in these patients. Furthermoreclinical trial research is required to validate these findings and explore the full therapeutic potential of NM in the treatment of diabetes.