Effects of Beta aminobutyric acid (BABA) on the gene expression profiles of rats with CCl4-induced Hepatic injury

Abstract

Background: The Liver plays a crucial role in regulating metabolic function and various physiological processes, it participates in the detoxification of medicines and xenobiotics, resulting in heightened susceptibility to the toxicity of these substances. The present study investigated the hepatotoxic effects of oxidative stress by analyzing the expression of the mir210, mir-34a, and mir146a genes in Thirty-four male Sprague–Dawley rats aged 8–10 weeks carbon tetrachloride-induced hepatotoxicity. Hepatic damage was stimulated in the groups (T1, T2, and T3) using CCL4. Beta aminobutyric acid (BABA) was added for five weeks at two different concentrations. T4, was treated with only CCL4 twice a week for five weeks, T5, which was divided into groups A, B, and C, was given BABA at different concentrations (100, 150, 200). The mean levels of the MIR34 34, MIR210, mIR146a, CD4 and CD 8 genes were evaluated.

Results: MIR34, MIR210 and MIR146a presented high mean levels in the T4 group (8.76, 2.67, 1.41), whereas MIR34 presented low levels in the control group (1.28±0.972), MIR210 in the T2 group (0.53) and MIR146a in the T5C group (0.56). CDs 4 and 8 presented high mean levels in the T4 group (11.53, 613.25), whereas CD 8 presented low levels in the control group (342.0), and CD 4 presented high mean levels in the T3 group (2.13). The results of MIR34 gene expression folding were (6.433, 5.282, 5.269, 8.364, 5.365, 2.990, 2.033, and 1.000) in the studied groups (T1, T2, T3, T4, T5A, T5B, T5C, and T6), the results of MIR34 gene expression according to the fold change were (1.613, 0.530, 0.951, 2.513, 1.634, 1.273, 0.549, and 1.004) in the studied groups (T1, T3, T4, T5A, T5B, T5C, and T6), and the results of MIR146a gene expression according to the fold change were (0.992, 0.419, 0.812, 1.807, 0.633, 0.638, 0.694, and 1.004) in the studied groups (T1, T5C, T4, T5A, T5B, T5C, and T6).

Conclusions: Compared with the other groups, the group injected with CCL4 presented high means of gene expression and greater fold changes in gene expression, which indicates that CCL4 causes severe damage to the rats.