Molecular Identification of Providencia spp .in birds and their owners in Baghdad, Iraq

Abstract

This study was conducted in  different farms of birds to isolate Providencia spp  from birds and their owners , A total of 100 samples were collected from different types of birds from cloacal swab of birds include pigeons (25), chickens (25) ,ducks(25), pet birds (15), parrots (10 ) collected from farms of bird and (50) stool samples from owners in  different  places in Baghdad city , Iraq. samples were inoculated in peptone water then cultured in Blood and MacConkeyagar. Identification of isolates was confirmed by conventional  methods include (microscopic examination, cultural characteristic, biochemical tests), Vitek2, and 16S rRNA  with sequencing. of 150 samples only  five samples positive 5/150 (3.3%) and P. vermicola were the 2/5(0%),  P. stuartii  3/5(60%) and number of  isolate were in chickens 1/5(20%),in ducks 1/5(40%),in pet birds 1/5(20%)  and in pigeons 1/5(20%) and in owners 1/5(20%).our results findings indicate that dropping  of birds  might be contaminated with Providencia spp.and this may  leading to zoonosis  transmission to all contact with birds   causing infection like  poisoning, diarrhea, urinary tract infections and other infections