Effect of Proteus Lipopolysaccharide (LPS) on virulence factor of Proteus mirabilis

Abstract

Two hundred samples were collected from different samples (urine, ear and wounds
swaps), from AL-Ramadi Teaching Hospital, Ramadi Maternity & Children Teaching Hospital.
The results of cultural, microscopic, biochemical tests indicated that in urine samples K.
pneumonia have high occurrence frequency 35 (29%) followed by Proteus mirabilis26(21.7%) and
Pseudomonas aeruginosa 20 (16.7%),while in wounds samples Pseudomonas aeruginosa10(25%)
followed by Klebsiella pneumonia5 (12.5%) and Proteus mirabilis 3 (7.5%) , while Ear swaps samples
revealed that P.aeruginosa 12(30%) was the major bacterium followed by P.mirabilis 4(10%)and
Klebsiella pneumonia 2(5%) . Sensitivity test against twelve antimicrobial agents was done for all
of the Proteus mirabilis isolates (33 isolates).The results displayed that most of the isolates were
resistant to Nitrofurantion (100%) ,Cefepime 70% followed by each Trimethoprim Sulfamethoxazole(60.6%) and Ceftriaxone(60.6%) while the most effective antimicrobial agent against P.mirabilis were Amoxicillin+Clavulanic acid (96.97%), Ofloxacin (78.79%),
Levofloxacin (78.78 %) Ciprofloxacin (69.7%),
Amikacin (66.7%), Imipenem (66.67%), Gentamicin (54.5%) and Naldixic acid (54.55%). More
resistant isolate was selected, and lipopolysaccharide was extracted by hot Phenol method this
method was tedious, but yielded LPS of high purity and the yield was (93) mg LPS from (5)g dry
weight cell of P.mirabilis After partial purification, chemical analysis of crud and partial purified
LPS showed that the amount of carbohydrate and protein in crude and partial purified LPS
extract were (69.375)mg ml-1, and (7.107)mg ml-1) respectively followed by, while the Carbohydrate in dialysis (85975 mg ml-1) (1.21mg ml-1) and in partial purification of LPS (93.011mg ml-1) while protein 0.144mg ml-1
.