Isolation Of Resistant Staphylococcus Aureus (MRSA) From Gingivitis And Assessment The Activity Of Propolis Loaded Lipid Nanoparticles Against Isolates

Abstract

Objective: Gingivitis is an inflammatory condition of the gingival tissue or the gums most commonly due to a bacterial infection . Methods: 154 samples from mouth cavity had been collected from patients who had Gingivitis.  The Samples were obtained from two sites, Teiba specialized medical center and the specialized center in Wasit City, Iraq. During the period from October ,2024 to March,2025. then methicillin-resistant Staphylococcus aureus were isolated by traditional methods . High-shear homogenization and ultra-sonication methods were used to prepare LNPs-PL. The manufactured formulation's encapsulation efficiency, loading capacity, and physicochemical characteristics were evaluated. Scanning electron microscopy (SEM) was used to analyze the morphology of LNPs-PL (SEM). Using GC/MS analysis, the chemical composition of the tested normal oil and LNPs-PL was assessed. In vitro studies were conducted to compare the antibacterial activity of LNPs-PL and regular PL.

Results:  the prevalence  of MRSA isolates were 37(58.73%) .the result of The antimicrobial activity of LNPs-PL showed increase the level of inhibition on the MRSA bacteria to (36 mm) comparative to (14 mm) with propolis (PL) Highly significant difference (P<0.01). LNPs-PL showed significantly larger inhibition zones vs. PL.

Conclusion: Based on the results of the study, modifications should be made in the loading capacity, encapsulation efficiency, and physicochemical properties. Our findings confirmed that SLNs are effective PL carriers for controlling bacterial and fungal diseases and further research is needed on them.