Optimizing Uv Spectrophotometry: A Reliable Method For 2-Hydroxybenzoic Acid Analysis In Face Serum

Abstract

Objectives: The objective of present work is to develop and standardize UV-Spectrophotometric method for the estimation of 2-Hydroxybenzoic Acid in marketed formulation.

Materials and Methods: Ultara Voilet-Spectrophotometric method was developed using Methanol as solvent. The developed method was standardized in terms of validation parameters such as specificity, selectivity, linear range, precision, robustness, ruggedness and reproducibility as per ICH (International Council for Harmonisation) guidelines. Newly developed and standardized method was successfully applied for estimation of 2-Hydroxybenzoic Acid in marketed formulation.

Results: 2-Hydroxybenzoic Acid exhibits λmax at 300nm and beer’s law was obeyed in the concentration range of 10 to 50µg/ml and limit of quantification is found to be mg/ml. The limit of detection found to be 1.20m25 Recovery of 2-Hydroxybenzoic Acid in marketed formulation was observed in the range of 90-110%. All the precision and repeatability results were within acceptance range less than 2%. Assay of 2-Hydroxybenzoic Acid was found to be 78.25%

Conclusion: The method was found to be simple, accurate, environment friendly, reproducible and can be used for routine estimation analysis of 2-Hydroxybenzoic Acid in marketed formulation.